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Published on: March 24, 2011
Expression of ASK1 during chick and early mouse development.
Anna Ferrer-Vaquer1, Pablo Maurey, Nicole Firnberg
1Developmental Biology Unit, Institute of Biology I, Faculty of Biology, University of Freiburg, Hauptstrasse 1, D-79104 Freiburg, Germany.
Gene Expression Patterns : GEP
|July 3, 2007
Summary
Apoptosis signal-regulating kinase 1 (ASK1) plays a role in cell death. This study reveals ASK1
Area of Science:
- Developmental Biology
- Cell Signaling
- Molecular Biology
Background:
- Apoptosis signal-regulating kinase 1 (ASK1) is a key regulator of stress-induced cell death pathways, including JNK and p38.
- While its pro-apoptotic role is established, ASK1's function during early embryonic development remains largely uncharacterized.
Purpose of the Study:
- To investigate the spatiotemporal expression pattern of chick ASK1 during early embryonic development.
- To compare chick ASK1 expression with its mouse counterpart.
- To elucidate the role of FGF signaling in regulating ASK1 expression in chick and mouse embryos.
Main Methods:
- Cloning of chick ASK1.
- Expression analysis of chick ASK1 from gastrulation (HH4) to day 4 of development using various techniques.
- Comparative analysis of ASK1 expression in chick and mouse embryos.
- Functional studies involving FGF signaling manipulation.
Main Results:
- Chick ASK1 was identified and cloned, with expression observed from gastrulation through day 4 in developing organs and limbs.
- Chick ASK1 expression in the nasal mesenchyme is dependent on FGF signaling.
- In contrast, mouse ASK1 expression in the nasal region is restricted to the epithelium and is FGF-independent.
- ASK1 exhibits conserved and species-specific expression domains in both chick and mouse embryos.
Conclusions:
- ASK1 displays dynamic and spatially restricted expression patterns during avian and murine embryonic development.
- FGF signaling differentially regulates ASK1 expression in the developing nasal region between chick and mouse.
- These findings highlight conserved and divergent roles of ASK1 in embryonic development across species.
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