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Bioreactor design for cornea tissue engineering: Material-cell interactions.

Elizabeth Orwin1, Ajay Shah, Andrew Voorhees

  • 1Harvey Mudd College, Claremont, CA, USA. orwin@hmc.edu

Acta Biomaterialia
|July 3, 2007
PubMed
Summary
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Poly(etheretherketone) and titanium-6Al-4V are suitable for corneal bioreactor systems. Other materials like poly(oxymethylene) copolymer and poly(tetrafluoroethlylene) are unsafe for human corneal fibroblasts after autoclaving.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Ophthalmology

Background:

  • Developing functional tissue-engineered corneas requires biocompatible materials for bioreactor systems.
  • Evaluating material safety and efficacy is crucial for clinical translation.

Purpose of the Study:

  • To assess the cytotoxicity of various materials intended for use in a tissue-engineered cornea bioreactor.
  • To identify the most suitable materials for supporting human corneal fibroblast viability and growth.

Main Methods:

  • Performed ASTM F813-01 24-hour cytotoxicity tests and 7-day growth inhibition assays.
  • Utilized human corneal stromal fibroblasts to evaluate material biocompatibility.
  • Assessed the impact of culture configuration, autoclaving, and surface preparation on cell viability.

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Main Results:

  • Poly(etheretherketone) (PEEK) and titanium-6Al-4V demonstrated the best biocompatibility for corneal bioreactor applications.
  • Poly(oxymethylene) copolymer and poly(tetrafluoroethlylene) exhibited cytotoxicity towards human corneal fibroblasts post-autoclaving.
  • Culture conditions, autoclaving, and material surface preparation significantly influenced cell viability.

Conclusions:

  • PEEK and titanium-6Al-4V are recommended materials for corneal bioreactor systems.
  • Poly(oxymethylene) copolymer and poly(tetrafluoroethlylene) are unsuitable for this application after autoclaving.
  • Material processing and preparation are critical factors in ensuring biocompatibility for ocular tissue engineering.