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Updated: Jul 14, 2026

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
Published on: November 11, 2016
Circadian rhythm of apoprotein H (beta2-glycoprotein-1) in human plasma
Masashi Higashimoto1, Yoshimi Homma, Miyuki Umetsu
1Department of Surgery II, Fukushima Medical University School of Medicine, Hikarigaoka-1, Fukushima, Japan.
Abstract:
Many physiological, biochemical, and behavioral processes are under circadian regulation, which is generated by an internal time-keeping mechanism referred to as biological clock. The regulators of circadian rhythm in human plasma have not been completely elucidated. Here we demonstrated that the isolated protein from human plasma, which down-regulated expression level of cry1 in Jurkat cells, was apoprotein H (ApoH). Further, mRNA expression level of ApoH indicated circadian rhythm in Jurkat cells. The concentration of ApoH was subject to circadian rhythm in human plasma. These experimental results suggested that ApoH may be one of the members of the regulator of circadian rhythm and that ApoH expression level was also dependent on circadian rhythm in Jurkat cells and in human plasma.
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