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Effect of storage on microcytosis observed in dogs with portosystemic vascular anomalies

Mark Goodfellow1, Kostas Papasouliotis, Simon Cue

  • 1Division of Companion Animal Studies, Department of Clinical Veterinary Science, Langford House, Langford, Bristol BS40 5DU, UK. m.goodfellow@bris.ac.uk

Research in Veterinary Science
|July 3, 2007
PubMed

Insights

Blood storage significantly alters red blood cell size in dogs. This change can mask microcytosis, a key indicator of portosystemic vascular anomalies (PSVA), in affected canine patients.

Area of Science:

  • Veterinary Hematology
  • Canine Pathophysiology

Background:

  • Microcytosis is a common finding in dogs with iron deficiency and congenital portosystemic vascular anomalies (PSVA).
  • Artefactual changes during blood storage can potentially mask microcytosis, complicating diagnosis.

Purpose of the Study:

  • To evaluate the impact of blood storage on microcytosis in dogs with congenital PSVA.
  • To determine if storage-induced changes affect the detection of microcytosis in affected dogs.

Main Methods:

  • Hematological parameters were measured in unaffected (n=13) and PSVA-affected (n=24) dogs.
  • Measurements were taken on the day of sampling and after 24 hours of storage at room temperature.

Main Results:

  • Blood storage for 24 hours significantly increased Mean Corpuscular Volume (MCV) in both groups (P<0.01).
  • The percentage increase in MCV was significantly greater in control dogs compared to PSVA-affected dogs (P<0.02).

Conclusions:

  • Storage of EDTA blood samples at ambient temperature for 24 hours significantly affects MCV.
  • These artefactual changes can mask the presence of microcytosis in dogs with PSVA, impacting diagnostic accuracy.

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