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Updated: Jul 14, 2026
Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
Published on: January 5, 2011
Milligram scale parallel purification of plasmid DNA using anion-exchange membrane capsules and a multi-channel
Per-Olof Syrén1, Aleksei Rozkov, Stefan R Schmidt
1Global Protein Science and Supply, AstraZeneca R&D Södertälje, SE-15185 Södertälje, Sweden.
Abstract:
A parallel chromatographic procedure for the purification of milligram amounts of plasmid DNA was developed. Initial studies showed that ion-exchange membrane capsules displayed high capacity for plasmid DNA. Interestingly, a weak anion exchanger (DEAE) proved to be superior to the strong quarternary ammonium group with respect to elution and regeneration properties and the 75 cm(2) Sartobind D membrane capsule (MA75D, Sartorius) was selected for further studies. A method for reducing endotoxin levels by using CTAB as a precipitant was optimised. By introducing this step into the protocol, endotoxin levels could be reduced approximately 100-fold to
