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Updated: Jul 14, 2026

Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells
Published on: May 19, 2020
Sequential polymicrobial infections lead to CNS inflammatory disease: possible involvement of bystander activation in
Ikuo Tsunoda1, Jane E Libbey, Robert S Fujinami
1Department of Neurology, University of Utah School of Medicine, 3R330 SOM, Salt Lake City, Utah 84132-2305, United States.
Abstract:
VV(PLP) is a recombinant vaccinia virus (VV) encoding myelin proteolipid protein (PLP) that has been used to investigate molecular mimicry and autoimmunity. Since virus infections can cause bystander activation, mice were first infected with VV(PLP), and later challenged with wild-type VV, lymphocytic choriomeningitis virus (LCMV), or murine cytomegalovirus (MCMV). Among the VV(PLP)-primed mice, only MCMV challenge induced significant Ki-67(+), CD3(+)T cell infiltration into the central nervous system (CNS) with a mild PLP antibody response. While MCMV alone caused no CNS disease, control VV-infected mice followed with MCMV developed mild CNS inflammation. Thus, heterologous virus infections can induce CNS pathology.
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