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Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
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Published on: January 11, 2017

A temperature-sensing histidine kinase: function, genetics, and membrane topology.

Yvonne Braun1, Angela V Smirnova, Helge Weingart

  • 1School of Engineering and Science, International University Bremen, Bremen, Germany.

Methods in Enzymology
|July 5, 2007
PubMed
Summary

Bacterial two-component systems help bacteria adapt to changing environments. Researchers found that the CorS protein in Pseudomonas syringae acts as a molecular thermometer, controlling toxin production based on temperature.

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Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Molecular Biology

Background:

  • Two-component systems are crucial for bacterial adaptation to environmental cues.
  • Many bacterial virulence factors are regulated by these systems, making them targets for combating infections.
  • Pseudomonas syringae pv. glycinea PG4180 produces the phytotoxin coronatine in a temperature-dependent manner.

Purpose of the Study:

  • To elucidate the temperature-sensing mechanism of the modified two-component system (CorRSP) regulating coronatine biosynthesis in Pseudomonas syringae pv. glycinea PG4180.
  • To understand how environmental temperature influences bacterial virulence factor production.

Main Methods:

  • Genetic analysis (mutagenesis)
  • Transcriptional analysis (mRNA quantification, reporter gene assays)
  • Biochemical analysis (protein expression, membrane topology)
  • In vitro and in planta experiments

Main Results:

  • The CorRSP system regulates temperature-dependent coronatine biosynthesis.
  • The histidine protein kinase CorS exhibits temperature-sensing activity.
  • CorS functions as a membrane-bound molecular thermometer.

Conclusions:

  • The CorRSP system, particularly CorS, plays a key role in sensing temperature to control phytotoxin production in Pseudomonas syringae.
  • Understanding this mechanism provides insights into bacterial virulence regulation and potential targets for intervention.