Single-cell analysis of gene expression by fluorescence microscopy
Related Experiment Video
Updated: Jul 13, 2026
10:50
Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
Published on: February 25, 2017
1Department of Physics, University of Pennsylvania, Philadelphia, PA, USA.
Methods in Enzymology
|July 5, 2007
Abstract:
Gene regulation by two-component systems has traditionally been studied using assays that involve averages over large numbers of cells. Single-cell measurements of transcription offer a complementary approach that provides the distribution of gene expression among the population. This chapter focuses on methods for using fluorescence microscopy and fluorescent proteins to study gene expression in single cells.
