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Daunorubicin impairs 1-beta-D-arabinofuranosylcytosine uptake
O Dal Pozzo1, P A Bernabei, L Lombardini
1UO Ematologia, Università degli Studi-USL 10/D, Firenze, Italia.
Chemotherapy
|January 1, 1991
Abstract:
Daunorubicin (DNR) and 1-beta-D-arabinofuranosylcytosine (Ara-C) act in a schedule-dependent fashion on leukemic cells. The present study was undertaken to determine whether the inhibition exerted by DNR on Ara-C cytotoxicity on HL 60 cells could be accounted for by an impairment of its uptake. After 250 ng/ml DNR treatment for 30 min, the time course of Ara-C uptake was measured, up to 128 min of incubation at 37 degrees C and at 4 degrees C, compared with controls. We found that DNR inhibits Ara-C uptake both at 37 and 4 degrees C, mostly acting on passive diffusion.