Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Performance assessment of diffuse optical spectroscopic imaging instruments in a 2-year multicenter breast cancer trial

Journal of biomedical optics·2018
Same author

Plasma Neutrophil Elastase and Elafin as Prognostic Biomarker for Acute Respiratory Distress Syndrome: A Multicenter Survival and Longitudinal Prospective Observation Study.

Shock (Augusta, Ga.)·2017
Same author

Simultaneous two-color stimulated Raman scattering microscopy by adding a fiber amplifier to a 2 ps OPO-based SRS microscope.

Optics letters·2017
Same author

Emission Flux Measurement Error with a Mobile DOAS System and Application to NO<sub>x</sub> Flux Observations.

Sensors (Basel, Switzerland)·2017
Same author

Regulatory Roles of Anoctamin-6 in Human Trabecular Meshwork Cells.

Investigative ophthalmology & visual science·2017
Same author

Prognostic significance of positive peritoneal cytology in resectable pancreatic cancer: a systemic review and meta-analysis.

Oncotarget·2017

Related Experiment Video

Updated: Jul 13, 2026

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
09:25

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

Published on: August 22, 2018

Method for recovering quantitative broadband diffuse optical spectra from layered media.

Ang Li1, Richard Kwong, Albert Cerussi

  • 1Beckman Laser Institute and Medical Clinic, University of California-Irvine, 92612, USA.

Applied Optics
|July 5, 2007
PubMed
Summary

This study demonstrates accurate recovery of optical properties and chromophore concentrations in a two-layer phantom. The diffuse optical methods precisely measure tissue layer characteristics, crucial for biomedical applications.

More Related Videos

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis
13:31

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

Published on: December 22, 2015

Related Experiment Videos

Last Updated: Jul 13, 2026

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
09:25

Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

Published on: August 22, 2018

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis
13:31

High Speed Sub-GHz Spectrometer for Brillouin Scattering Analysis

Published on: December 22, 2015

Area of Science:

  • Biomedical Optics
  • Photonics and Spectroscopy
  • Tissue Optics

Background:

  • Diffuse optical spectroscopy is vital for non-invasive tissue analysis.
  • Accurate characterization of layered tissue phantoms is essential for validating optical methods.
  • Understanding chromophore concentrations and layer thickness is key in biomedical imaging.

Purpose of the Study:

  • To develop and validate an algorithm for recovering broadband diffuse optical properties from a two-layer phantom.
  • To assess the accuracy of chromophore concentration and layer thickness measurements.
  • To evaluate the algorithm's robustness to initial parameter guesses.

Main Methods:

  • Utilized broadband (650-1000 nm) diffuse optical spectroscopy.
  • Employed a two-layer phantom simulating adipose and muscle tissues.
  • Applied a novel algorithm to recover optical properties and layer thickness.

Main Results:

  • Achieved average errors of 10% for absolute chromophore concentrations and 3% for dynamic variations.
  • Successfully recovered top layer thickness up to 12 mm with <10% error.
  • Demonstrated algorithm insensitivity to initial optical property guesses when thickness guess is within +/-2 mm.

Conclusions:

  • The developed algorithm accurately quantifies optical properties and chromophore concentrations in layered phantoms.
  • This method shows promise for non-invasive in vivo tissue analysis.
  • Robustness to initial guesses enhances practical applicability in diffuse optical tomography.