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Mosaic genome structure of echovirus type 30 that circulated in Taiwan in 2001

G-W Chen1, J-H Huang, Y-L Lo

  • 1Department of Computer Science and Information Engineering, Chang Gung University, Taoyuan, Taiwan.

Archives of Virology
|July 5, 2007
PubMed

Insights

Molecular typing identified echovirus type 30 (E30) during a 2001 Taiwan outbreak, despite initial misidentification. Analysis revealed recombinant E30 strains with mosaic genomes, suggesting frequent recombination events.

Area of Science:

  • Virology
  • Molecular Biology
  • Epidemiology

Background:

  • Echovirus type 30 (E30) outbreaks pose public health concerns.
  • Accurate identification of viral strains is crucial for outbreak management.

Purpose of the Study:

  • To genetically characterize enterovirus isolates from a 2001 Taiwanese meningitis outbreak.
  • To investigate discrepancies in viral identification using serological and molecular methods.

Main Methods:

  • Genetic analysis of enterovirus isolates from cerebrospinal fluid (CSF).
  • Molecular typing using partial VP1 sequences.
  • Neutralization tests and immunofluorescent assays (IFA) with specific monoclonal antibodies.
  • Complete genome sequencing and recombination analysis.

Main Results:

  • Twenty enterovirus isolates were identified as E30 through molecular typing, despite initial negative results with E30-specific antibodies.
  • Seven isolates were misidentified as echovirus type 4 (E4) using E4-specific antibodies.
  • Complete genome analysis revealed two 2001 Taiwanese E30 strains with mosaic structures, indicating probable recombination.
  • Significant nucleotide divergence was observed between structural (P1) and nonstructural (P2, P3) regions, supporting recombinant origins.

Conclusions:

  • Molecular methods are essential for accurate identification of E30, especially during outbreaks.
  • The 2001 Taiwanese E30 strains exhibit evidence of genetic recombination, suggesting ongoing viral evolution.
  • Mosaic genome structures may arise from frequent recombination events in enteroviruses.

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