Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Laser capture-microarray analysis of Lim1 mutant kidney development.

S Steven Potter1, Heather A Hartman, Kin M Kwan

  • 1Division of Developmental Biology, Children's Hospital Medical Center, Cincinnati, Ohio 45229-3039, USA. steve.potter@cchmc.org

Genesis (New York, N.Y. : 2000)
|July 5, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Child access prevention laws and firearm storage in the US: Associations by law stringency and social vulnerability" Preventive Medicine Volume 204 (2026), 108516.

Preventive medicine·2026
Same author

Comparative Effectiveness of Antihypertensive Medications in Children With Chronic Kidney Disease.

JAMA pediatrics·2026
Same author

Child access prevention laws and firearm storage in the US: Associations by law stringency and social vulnerability.

Preventive medicine·2026
Same author

Oogenesis and germinal bed morphology of the brown anole (A. sagrei).

Developmental dynamics : an official publication of the American Association of Anatomists·2026
Same author

A long non-coding RNA Leat1 mediates the hormone responsiveness of EfnB2 during male urogenital development.

Communications biology·2025
Same author

Poly(A) probe HCR RNA-FISH specifically marks pyriform nurse cells in the brown anole lizard ovary.

Developmental biology·2025
Same journal

Meet Our Editorial Board-Genesis. An Interview With Anna Di Gregorio, New York University, New York, USA.

Genesis (New York, N.Y. : 2000)·2026
Same journal

Multiplexed Retinoic Acid Signal Reporters for In Vivo and In Vitro Use.

Genesis (New York, N.Y. : 2000)·2026
Same journal

Generation and Characterization of Twist1 Acetyl-Mimic and Acetyl-Deficient Mouse Models.

Genesis (New York, N.Y. : 2000)·2026
Same journal

Phylogenetic Comparison and Splice Site Conservation of the Animal SMNDC1 Gene Family.

Genesis (New York, N.Y. : 2000)·2026
Same journal

Pvr and Pvf2 Are Essential for Valve Cell Differentiation in the Larval Drosophila Heart.

Genesis (New York, N.Y. : 2000)·2026
Same journal

Efficient Gene Expression System in Medaka Embryos Enables Functional Characterization of nt5c1a Paralogs Involved in Inosine Monophosphate Metabolism.

Genesis (New York, N.Y. : 2000)·2026
See all related articles

Lim1 gene is crucial for kidney development, halting nephron formation at the renal vesicle stage in mutants. Downregulation of Dkk1, a Wnt signaling inhibitor, suggests it

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • The Lim1 gene plays a critical role in kidney development.
  • Specific Lim1 knockout in metanephric mesenchyme prevents renal vesicle to comma-shaped body transition.

Purpose of the Study:

  • Investigate the molecular mechanisms behind the developmental arrest caused by Lim1 deficiency.
  • Identify downstream targets of Lim1 function in early kidney development.

Main Methods:

  • Laser capture microdissection coupled with microarray analysis to study gene expression in mutant renal vesicles.
  • In situ hybridization to validate microarray findings.

Main Results:

  • Identified differentially expressed genes including Chrdl2, Bmf, myob5, and pdgfrl.

Related Experiment Videos

  • Observed a ninefold downregulation of Dkk1, a Wnt signaling inhibitor, in Lim1 mutant renal vesicles.
  • Confirmed Dkk1 downregulation using in situ hybridization.

    • Conclusions:

    • Dkk1 may function as a key downstream effector of Lim1 in kidney development.
    • The observed similarities between Lim1 and Dkk1 mutant mouse phenotypes support this hypothesis.