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Related Experiment Videos

Generation of a conditional Ppap2b/Lpp3 null allele.

Diana Escalante-Alcalde1, Roberto Sánchez-Sánchez, Colin L Stewart

  • 1Cancer and Developmental Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, Maryland, USA.

Genesis (New York, N.Y. : 2000)
|July 5, 2007
PubMed
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Lipid phosphohydrolase Lpp3 (Pap2b) is crucial for embryonic development. Conditional gene inactivation confirmed its essential role in vascular development and gastrulation, mirroring null allele phenotypes.

Area of Science:

  • Biochemistry
  • Developmental Biology
  • Genetics

Background:

  • Lpp3 (Pap2b) is a lipid phosphohydrolase.
  • Its substrates and products include signaling lipids like phosphatidic acid and sphingosine-1-phosphate.
  • Lpp3 is dynamically expressed during development and in adult tissues.

Purpose of the Study:

  • To investigate the role of Lpp3 in later development and specific cell lineages.
  • To generate a conditional allele of the Ppap2b gene for targeted inactivation.

Main Methods:

  • Generated a conditional Ppap2b allele by flanking critical exons with loxP sites.
  • Utilized Cre-mediated recombination to achieve gene inactivation.

Main Results:

Related Experiment Videos

  • Conditional inactivation of Ppap2b resulted in embryonic lethality.
  • Observed defects in extraembryonic vascular development and gastrulation.
  • The phenotype was identical to the previously reported Ppap2b null allele.

    • Conclusions:

    • Lpp3 is essential for embryonic vascular development and gastrulation.
    • The conditional allele effectively recapitulates the null phenotype, enabling lineage-specific studies.
    • Lpp3 plays a critical role throughout embryonic development.