Overground locomotion after incomplete spinal lesions in the rat: quantitative gait analysis

In rats with incomplete low thoracic spinal cord lesions of different extents, the basic indices of gait such as locomotor velocity, step and stance phase duration and the duty factor (i.e., the relative duration of the stance phase) during overground runway locomotion were analyzed using contact electrodes on each paw for data recording. In animals with lesions confined to the dorsal columns (DC), tested 3 weeks postsurgery, these gait indices were essentially unchanged compared to the preoperative period. After the same recovery period, rats with larger lesions, comprising the dorsal columns plus a major part of the dorsolateral funiculi (DL), showed a transient increase in the hindlimb stance phase duration and the duty factor. More extensive injuries, with additional damage to parts of the ventrolateral and ventral funiculi (VL), produced increments in the stance phase duration and duty factor much above that which would be expected from changes in step cycle duration due to slowing down of locomotion. These changes, which lasted for at least 3 months, were more conspicuous in animals with extensive spinal cord injuries and were due to an altered relationship between the stance phase and step cycle duration. It is suggested that the excessive increment in the hindlimb stance phase and the duty factor constitute a reliable indicator of impairment in locomotor movements, which is correlated with the extent of spinal cord injury.