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Updated: Jul 13, 2026

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
Published on: April 23, 2016
Deletion of entire HLA-A gene accompanied by an insertion of a retrotransposon
M Takasu1, R Hayashi, E Maruya
1Department of Human Genetics, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
Abstract:
Unusual HLA-A'null' alleles because of an entire gene deletion were found in three apparently unrelated Japanese families with leukemia patients. Inclusion of the entire HLA-A gene in the deletion was confirmed by polymerase chain reaction direct sequencing of the surrounding regions of HLA-A. Further localization of the breakpoints of the HLA-A deletion at the centromeric and telomeric sides was performed, and these families were shown to possess the identical deletion. We then determined the genomic sequence of the HLA-A-deleted haplotype. Surprisingly, the haplotype turned out to carry an insertion of an SVA (SINE-VNTR-Alu) retrotransposon of 2 kb as well as the 14 kb deletion that included the entire HLA-A gene.
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