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Heterogeneity of synaptosomal membrane preparations from different regions of calf brain studied by partitioning and
J A Cebrian Pérez1, M T Muiño Blanco, G Johansson
1Department of Biochemistry, University of Lund, Sweden.
Abstract:
1. Membranes obtained by lysis and Yeda-press treatment of synaptosomes (nerve endings) from cortex, caudateus nucleus, and hippocampal region of calf brain have been studied by partitioning within a liquid-liquid aqueous two-phase system consisting of water, dextran, Ficoll, and poly(ethylene glycol). 2. The partitioning of membranes was sensitive to the presence of a dextran-bound dye, Procion yellow HE-3G, in the lower phase. 3. The two-phase system was used for counter-current distribution to study the heterogeneity of the synaptic membranes from the three regions of the brain and to separate the membranes into fractions. 4. The obtained counter-current distribution profiles strongly depended on the region of the brain from which the membranes were isolated. 5. The membrane fractions obtained showed marked differences in their SDS electrophoresis pattern.