Related Experiment Video
Updated: Jul 13, 2026

10:31
A Model Membrane Platform for Reconstituting Mitochondrial Membrane Dynamics
Published on: September 2, 2020
Characterization of cake layer in submerged membrane bioreactor
Fangang Meng1, Hanmin Zhang, Fenglin Yang
1School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, People's Republic of China.
Environmental Science & Technology
|July 7, 2007
Summary
Membrane bioreactors (MBRs) face challenges with cake layer formation. This study reveals that small sludge particles, bacterial clusters, polysaccharides, and inorganic elements like Mg and Ca contribute significantly to membrane fouling.
Area of Science:
- Environmental Science
- Biotechnology
- Materials Science
Background:
- Cake layer formation on membranes is a significant operational challenge in membrane bioreactors (MBRs).
- Understanding the composition and formation mechanism of this cake layer is crucial for optimizing MBR performance in wastewater treatment.
Purpose of the Study:
- To investigate the cake layer formation mechanism in a membrane bioreactor used for synthetic wastewater treatment.
- To identify the major organic and inorganic components contributing to membrane fouling.
Main Methods:
- Particle size analysis (PSA)
- Scanning electron microscopy (SEM)
- Confocal laser scanning microscopy (CLSM)
- X-ray fluorescence (XRF)
- Energy-dispersive X-ray analysis (EDX)
- Fourier transform infrared (FTIR) spectroscopy
Main Results:
- Small particles in sludge suspension exhibit a strong tendency to deposit on the membrane surface.
- Bacterial clusters and polysaccharides are identified as major contributors to membrane fouling via SEM and CLSM.
- FTIR analysis confirms proteins and polysaccharides as key biopolymer components.
- EDX and XRF analyses reveal Mg, Al, Ca, Si, and Fe as the predominant inorganic elements in the fouling cake.
- Bridging between biopolymers and inorganic compounds enhances fouling layer compactness.
Conclusions:
- Effective control of biopolymers and inorganic elements within the bioreactor is essential for minimizing membrane fouling.
- The findings provide insights into mitigating operational challenges in MBR systems.
Related Concept Videos
Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Microbial Mats
Microbial communities forming biofilms and mats represent complex, spatially structured ecosystems where metabolic processes are stratified according to light, oxygen, and nutrient gradients. Biofilms are initial colonization stages, only a few millimeters thick, while mature microbial mats can reach centimeter-scale thickness and display intricate vertical organization. Their structural and functional heterogeneity allows microorganisms to occupy distinct ecological niches within a few...
Asymmetric Lipid Bilayer
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...

