Related Experiment Video
Updated: Jul 13, 2026
Dosimetry for Cell Irradiation using Orthovoltage (40-300 kV) X-Ray Facilities
Published on: February 20, 2021
Effect of radiation scattering on dose uniformity in open and closed cell culture vessels
Myonggeun Yoon1, Sung Yong Park, Jungwook Shin
1Radiation Oncology, Proton Therapy Center, National Cancer Center, Ilsandong-gu, Goyang, Korea.
Purpose:
Dose uniformity in cell culture vessels such as Petri dishes and anoxic irradiation chambers is very important in radiobiological work as dose uniformity affects cell survival probabilities. In this study, we investigated X-ray dose inhomogeneity, caused by scattering, in typical culture vessels.
Materials And Methods:
Three different cubic cell culture vessels, with side lengths of 10 cm, 15 cm and 20 cm, were designed and irradiated by X-rays of 6 MV and 15 MV at a source-to-surface distance (SSD) of 100 cm using a Varian 2,100CD linear accelerator.
Results:
The relative X-ray dose distribution in a cell culture vessel depended strongly on whether the vessel had a lid. The percentage of the cell culture surface with the dose differing by more than 10% from the mean value of the dose was 43.4% in lidless vessels and 9.7% in lidded vessels.
Conclusions:
In radiobiological work, X-ray dose inhomogeneity within a cell culture vessel is not negligible and the placement of cells in the vessel should be carefully considered.
