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Updated: Jul 13, 2026

Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia
Published on: June 8, 2019
Vascular assessment and reconstruction of the ischemic diabetic limb
1Division of Vascular and Endovascular Surgery, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA. haginor@uthscsa.edu
Abstract:
Peripheral artery disease (PAD) is a common finding among diabetic patients and is usually secondary to atherosclerosis. PAD in diabetic patients tends to be more malignant, presenting with diffuse involvement and more complications. Unless the ischemia is addressed concomitantly with local problems in the foot, resulting problems can lead to extensive tissue loss and major amputation. Lower extremity atherosclerotic complications in diabetic patients require considerable dedication and aggressiveness if limb loss is to be avoided.
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Position the patient at a thirty- to forty-five-degree angle or in a semi-fowler's position. Look for the highest point of pulsation in the internal jugular vein and measure the vertical distance to the angle of Loius or sternal angle. A normal JVP is 3-4 cm above the...

