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Updated: Jul 13, 2026

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
Published on: December 13, 2019
Distribution coefficient of rifabutin in liposome/water system as measured by different methods
Vitaly V Vostrikov1, Alla A Selishcheva, Galina M Sorokoumova
1M.V. Lomonosov State Academy of Fine Chemical Technology, Moscow, Russia. vvostri@uark.edu
Abstract:
Distribution coefficient (D) of rifabutin in liposome/water system was measured by phase separation and fluorescence probe quenching techniques. D values were identical suggesting that rifabutin is fully immersed into lipid bilayer. Structural studies of phospholipid bilayer employing (31)P NMR spectroscopy demonstrated that introduction of rifabutin does not alter the bilayer structure. A scheme of the rifabutin position in lipid bilayer based on the calculated size of rifabutin molecule is proposed.
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