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An alternative method for delivering exogenous material into developing zebrafish embryos.

Vikram Kohli1, Vanesa Robles, M Leonor Cancela

  • 1Ultrafast Photonics and Nano-Optics Laboratory, Centre for Nanoelectronics, Nanophotonics & Nanoscale Systems, Department of Electrical and Computer Engineering, University of Alberta, Edmonton, Alberta, Canada. vkohli@ece.ualberta.ca

Biotechnology and Bioengineering
|July 7, 2007
PubMed
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Femtosecond laser pulses create transient pores for non-invasive intracellular delivery in zebrafish embryos. This method successfully introduced molecules and DNA, demonstrating high embryo survival rates for biological research.

Area of Science:

  • Biotechnology
  • Developmental Biology
  • Laser Physics

Background:

  • Non-invasive manipulation of multicellular systems is crucial for biological and medical research.
  • Intracellular delivery of molecules is key to understanding cellular functions.

Purpose of the Study:

  • To report a novel method for introducing foreign material into developing embryos using femtosecond laser pulses.
  • To demonstrate the non-invasiveness and efficacy of laser-induced pore formation for intracellular delivery in zebrafish embryos.

Main Methods:

  • Application of intense femtosecond (fs) laser pulses focused to a sub-micron spot to create transient pores.
  • Delivery of exogenous materials including fluorescein isothiocyanate, quantum dots, and DNA into zebrafish embryos.
  • Assessment of pore formation at different embryonic locations and evaluation of long-term embryo survival.

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Main Results:

  • Optically induced transient pores facilitated the successful loading of various molecules and DNA into zebrafish embryos.
  • Pore formation was effective at both the blastomere-yolk interface and within blastomeres.
  • High survival rates were observed: 89% for dechorionated and 100% for chorionated embryos post-manipulation.

Conclusions:

  • Femtosecond laser pulses offer a non-invasive method for intracellular delivery in developing embryos.
  • This technique enables the delivery of diverse materials, including DNA, into zebrafish embryos.
  • The high survival rates indicate the potential of this method for future biological and medical applications.