Related Experiment Video
Updated: Jul 13, 2026

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments
Published on: July 24, 2018
[Genetic diversity of microbial communities in tea orchard soil]
Dong Xue1, Huai-Ying Yao, Chang-Yong Huang
1College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029, China. xuedong2004@zju.edu.cn
Abstract:
In this paper, the total microbial DNA was extracted from the soils in 8-, 50- and 90 years old tea orchards, adjacent wasteland, and 90 years old forestland in Meijiawu tea area of Hangzhou. The 16S rDNA V3 fragment was amplified by PCR, and the polymorphism of this fragment was analyzed by DGGE. The results indicated that both the tea orchard age and the land use type had significant effects on soil microbial genetic diversity. There was a significant difference (P < 0.05) in the microbial genetic diversity index among wasteland, tea orchards and forestland, which was decreased in the order of wasteland > tea orchard > forestland. For the tea orchards of different ages, the soil microbial genetic diversity index, microbial biomass C, and basal respiration were significantly higher in 50 years old than in 8 and 90 years old tea orchards.
Related Concept Videos
Soil Microbial Ecology
Methods to Assess Microbial Communities
Introduction to Microbial Ecology
Diversity of Archaea IV
Diversity of Archaea I
Diversity of Archaea III

