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Updated: Jul 13, 2026

Murine Model for Parkinson's Disease: from 6-OH Dopamine Lesion to Behavioral Test
Published on: January 15, 2010
Experimental change on dopaminergic neurons in striatum of Parkinson disease rats
1School of Pharmacy, Anhui Medical University, Hefei, China. chenxiaoyuzlx@yahoo.com.cn
Background:
Parkinson's disease (PD) is a common neurodegenerative disorder characterized by selective and progressive demise of dopamine-containing neurons in the midbrain. In this study, we observe the expression of c-Jun in the striatum of rats with 6-hydroxydopamine (6-OHDA)-lesions after apomorphine (APO) intraperitoneal injection (ip) in substantia nigra compacta (SNc), and to study the mechanism of the rotations behavior.
Design:
The 6-OHDA was unilaterally injected into rat right SNC. The APO- induced abnormal rotations were investigated on the 1st, 4th, 7th, 14th, 21st days after lesion, respectively. Meanwhile dopaminergic degeneration and c-Jun expression were observed with microscope. Nissl's body staining and immunohistochemical method (ABC) were employed to study the changes of tyrosine hydroxylase (TH) and c-Jun in DA neurons.
Results:
We found that the number of dopaminergic neurons decreased gradually in the lesioned site and those neurons' electron-microscopic structure was severe damaged. There were over 75% of dopaminergic neurons lost, contralateral rotations over 7 turns per minute and c-Jun expressing in the ipsilateral striatum.
Conclusion:
Dopaminergic neurons deletion may be linked to upregulation of c-Jun.
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