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Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

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Published on: February 21, 2018

Stat5 is essential for early B cell development but not for B cell maturation and function.

Xuezhi Dai¹, Yuhong Chen, Lie Di

¹State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, People's Republic of China.

Journal of Immunology (Baltimore, Md. : 1950)

|July 10, 2007

Summary

Signal transducer and activator of transcription 5 (Stat5A/5B) proteins are crucial for early B cell development. These proteins directly control IL-7-mediated B cell expansion but are not essential for B cell maturation or Ig production.

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Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

Area of Science:

Immunology
Molecular Biology
Hematopoiesis

Background:

Signal transducer and activator of transcription 5 (Stat5A and Stat5B) are activated by cytokines.
Their role in lymphocyte development, particularly B cells, remains incompletely understood.
Stat5A/5B involvement in stem cell function adds complexity to their study.

Purpose of the Study:

To elucidate the specific role of Stat5A/5B in B cell development and function.
To investigate the direct impact of Stat5A/5B on IL-7-mediated B cell expansion.
To differentiate the requirement of Stat5A/5B in early versus late B cell development.

Main Methods:

Analysis of Stat5A/5B(-/-) fetal liver cells in vitro and in vivo.
Transplantation of mutant fetal liver cells into irradiated mice.
In vitro IL-7-mediated B cell expansion assays.
Reintroduction of Stat5A into mutant cells.
CD19-Cre-mediated deletion of Stat5A/5B in the B cell compartment.

Main Results:

Stat5A/5B(-/-) fetal liver cells showed a severe reduction in B cell progenitors but normal myeloid progenitors.
Mutant cells could not develop B cells beyond the pro-B cell stage in vivo.
Stat5A/5B deletion impaired IL-7-mediated B cell expansion in vitro.
Reintroduction of Stat5A rescued B cell development.
B cell-specific Stat5A/5B deletion impaired early B cell development but not maturation or Ig production.

Conclusions:

Stat5A/5B proteins are essential for IL-7-mediated early B cell development.
Stat5A/5B are not required for B cell maturation, survival, proliferation, or Ig production.
These findings clarify the specific role of Stat5A/5B in the B cell lineage.