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Related Experiment Videos

Microfluidic systems integrated with two-dimensional surface plasmon resonance phase imaging systems for microarray

Kuo-Hoong Lee1, Yuan-Deng Su, Shean-Jen Chen

  • 1Department of Engineering Science, National Cheng Kung University, Taiwan.

Biosensors & Bioelectronics
|July 10, 2007
PubMed
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This study developed a microfluidic chip with surface plasmon resonance (SPR) phase imaging for sensitive and specific bio-detection. The system successfully identified rabbit immunoglobulin G (IgG) with a low detection limit, showing promise for diagnostics.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Optics

Background:

  • Surface Plasmon Resonance (SPR) is a label-free optical technique for detecting biomolecular interactions.
  • Microfluidic systems offer precise control over sample handling and reaction conditions.
  • Arrayed immunoassays enable high-throughput screening of multiple analytes simultaneously.

Purpose of the Study:

  • To develop an integrated microfluidic chip with an arrayed immunoassay for Surface Plasmon Resonance (SPR) phase imaging.
  • To achieve sensitive and specific detection of bio-samples, specifically rabbit immunoglobulin G (IgG).
  • To demonstrate the utility of the system for applications in medical diagnostics and protein-protein interaction studies.

Main Methods:

  • Fabrication of a microfluidic chip using micro-electro-mechanical-systems (MEMS) technology on glass and polydimethylsiloxane (PDMS) substrates.

Related Experiment Videos

  • Integration of an arrayed immunoassay with SPR phase imaging for 2D spatial phase variation detection.
  • Implementation of a micromachine-based temperature control module for stable detection conditions (<0.3°C variation).
  • Surface functionalization using self-assembled monolayers (SAM) to immobilize anti-rabbit IgG on a gold layer.

    • Main Results:

    • Successful detection of interactions between immobilized anti-rabbit IgG and rabbit IgG using SPR phase imaging.
    • Achieved a low detection limit of 1 x 10⁻⁴ mg/ml (0.67 nM) for rabbit IgG.
    • Demonstrated high specificity, with successful detection of rabbit IgG and no adsorption of porcine IgG.

    Conclusions:

    • The developed microfluidic chip integrated with SPR phase imaging provides a sensitive and specific platform for bio-sensing.
    • The system's precise sample delivery and stable temperature control contribute to reliable immunoassay performance.
    • This technology holds significant potential for applications in medical diagnostics, microarray detection, and protein-protein interaction analysis.