Related Experiment Video
Updated: Jul 13, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
Expression of Escherichia coli DcuS-R two-component regulatory system is regulated by the secondary internal promoter
Tomoya Oyamada1, Katsushi Yokoyama, Michiko Morinaga
1Department of Applied Chemistry, National Defense Academy of Japan, Yokosuka, Japan.
Abstract:
The DcuS-R two-component system of Escherichia coli senses C4-dicarboxylates of the medium and regulates expression of the genes related to utilization of them. It is known that phospho-DcuR induces expression of genes such as the dcuB-fumB operon, the frdABCD operon, and the dctA gene. We analyzed promoters of the dcuS-R operon to elucidate the transcriptional regulation system. We found a novel internal promoter within the dcuS gene that is regulated by the transcriptional regulator, CRP-cAMP, in both aerobic and anaerobic conditions.
Related Concept Videos
Global Regulatory Systems
Inducible Operons: lac Operon
Stringent Response in E. coli
Repressible Operon: trp Operon
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...

