Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...

Conservation of Protein Domains Over Different Proteins

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain. Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...

Cytoskeletal Linker Proteins - Plakins

Cytoskeletal Linker Proteins - Plakins

Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...

Cytoskeletal Accessory Proteins

Cytoskeletal Accessory Proteins

The cytoskeleton is an essential cell component that plays several structural and functional roles. However, the filaments that make up the cytoskeleton cannot function independently and depend on the accessory or ancillary proteins to effectively carry out their function. Accessory proteins associate with cytoskeletal filaments and their monomers, aiding filament formation and function. They also help in the cross-communication among cytoskeletal filaments. Cytoskeletal accessory proteins are...

The Replisome

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Molecular Confirmation of Autochthonous Taenia saginata Infection, Timor-Leste, 2019.

Emerging infectious diseases·2026

Same author

A LAMP-based assay for rapid detection of human scabies using clinical skin scraping specimens.

Scientific reports·2026

Same author

Ginsenoside Rd attenuates fine particulate matter-induced pulmonary inflammation by directly inhibiting the transforming growth factor-beta-activated kinase 1-mediated toll-like receptor 2 signaling.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026

Same author

The association between the consumption of raw Kudoa septempunctata-infected farmed Paralichthys olivaceus and gastrointestinal symptoms.

Epidemiology and health·2026

Same author

The in vitro digestive mechanism of Tartary buckwheat starch-flavonoid complexes treated with different methods.

Carbohydrate polymers·2025

Same author

Spotlight on psychological well-being and cyberbullying among teenagers and young adults: a systematic review of the literature.

BMC psychology·2025

Search research articles

Related Experiment Video

Updated: Jul 13, 2026

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

Partner proteins that interact with Clonorchis sinensis WD40-repeat protein.

Tae Im Kim¹, Pyo Yun Cho, Shunyu Li

¹Department of Parasitology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul, 156-756, South Korea.

Parasitology Research

|July 10, 2007

Summary

The Clonorchis sinensis WD40-repeat protein 1 (CsWD1) interacts with various proteins involved in intracellular transport and cell cycle control. This discovery sheds light on the function of CsWD1 in the parasite

More Related Videos

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

Enhanced Crosslinking Immunoprecipitation (eCLIP) Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Enhanced Crosslinking Immunoprecipitation (eCLIP) Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Published on: May 10, 2019

Related Experiment Videos

Last Updated: Jul 13, 2026

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

In Situ Detection of Ribonucleoprotein Complex Assembly in the C. elegans Germline using Proximity Ligation Assay

Published on: May 5, 2020

Enhanced Crosslinking Immunoprecipitation (eCLIP) Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Enhanced Crosslinking Immunoprecipitation (eCLIP) Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Published on: May 10, 2019

Area of Science:

Parasitology
Molecular Biology
Protein Interactions

Background:

WD40-repeat proteins are characterized by multiple repeat units and a beta-propeller fold.
The specific WD40-repeat protein from Clonorchis sinensis, CsWD1, is expressed during the metacercaria stage.

Purpose of the Study:

To identify proteins that interact with CsWD1.
To elucidate the functional roles of CsWD1 in Clonorchis sinensis metacercariae.

Main Methods:

Yeast two-hybrid screening was employed to identify interacting partners.
High-stringency screening and lacZ colony-lift assays were used for validation.
Nucleotide and polypeptide sequences were assessed for selected cDNAs.

Main Results:

Twelve putative interacting protein clones were confirmed to interact with CsWD1.
These interacting proteins were categorized into functional groups: signal proteins, transporters, proteases, and muscle proteins.

Conclusions:

CsWD1 protein is implicated in intracellular protein translocation.
CsWD1 plays a role in cell cycle control within Clonorchis sinensis metacercariae.