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Updated: Jun 29, 2026
Assessment of Human Natural Killer Cell Events Driven by FcγRIIIa Engagement in the Presence of Therapeutic Antibodies
Published on: May 22, 2020
[Leukemic cell apoptosis induced by anti-human DR5 monoclonal antibody mDRA-6]
Shu-lian Li1, Yuan-fang Ma, Guang-chao Liu
1Institute of Immunology, Laboratory of Cellular and Molecular Immunology, Henan University, Kaifeng 475004, China. lshl92627@henu.edu.cn
Aim:
To investigate the apoptotic effect of anti-human DR5 (death receptor 5 of TRAIL) monoclonal antibody mDRA-6 on leukemic cells.
Methods:
The morphological changes of leukemic cells were observed by fluorescence microscope. The cytotoxic and apoptotic effects of mDRA-6 on Jurkat, HL-60 and K562 cells were detected by MTT analysis and flow cytometry with Annexin V-FITC/PI staining.
Results:
Chromatin condensation, budding and apoptotic bodies were observed in Jurkat and HL-60 cells treated by mDRA-6. Death and apoptosis of leukemic cells treated by mDRA-6 were increased, but the effect of mDRA-6 on K562 cells was not obvious.
Conclusion:
Apoptosis of leukemic cells can be induced by anti-human DR5 monoclonal antibody mDRA-6. Different leukemic cell lines are of different sensitivity to mDRA-6.
