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Updated: Jul 13, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
Published on: August 16, 2019
Affinity-based methodologies and ligands for antibody purification: advances and perspectives.
Ana C A Roque1, Cláudia S O Silva, M Angela Taipa
1IBB-Institute for Biotechnology and Bioengineering, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal.
Novel antibody purification strategies are crucial for cost-effective therapies. This review highlights advances in affinity chromatography for efficient antibody separation and production.
Area of Science:
- Biotechnology and Pharmaceutical Sciences
- Immunology and Molecular Biology
Background:
- Therapies for cancer and rheumatoid arthritis rely on antibody-receptor recognition.
- The demand for specific, single-use antibodies necessitates advanced purification methods.
- Downstream processing significantly impacts the manufacturing cost and availability of antibody-based therapeutics.
Purpose of the Study:
- To review recent advancements in antibody purification techniques.
- To emphasize the role of affinity interactions, particularly affinity chromatography, in antibody separation.
- To discuss future perspectives in antibody purification strategies.
Main Methods:
- Review of scientific literature on antibody purification.
- Focus on affinity-based separation strategies.
- Analysis of various affinity ligands and chromatographic techniques.
Main Results:
- Affinity-based strategies are the most common and efficient for antibody purification.
- Development of diverse affinity ligands, including synthetic molecules, enhances stability and resistance.
- Continuous efforts are directed towards improving separation performance in antibody purification.
Conclusions:
- Advances in affinity chromatography offer improved antibody purification.
- Novel strategies are essential for reducing manufacturing costs and increasing therapeutic accessibility.
- Further innovation in purification techniques will drive the development of next-generation antibody therapies.
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