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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
A new monopartite begomovirus isolated from Hibiscus cannabinus L. in India
1Plant Virus Laboratory and Biotechnology Unit, Division of Crop Protection, Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata, India.
Abstract:
Yellow vein mosaic disease of mesta is whitefly-transmitted and found in endemic form in different parts of India, causing great economic losses. Full-length DNA-A of a begomovirus infecting mesta was cloned and sequenced. The genome was homologous to the DNA-A of monopartite begomoviruses originating from the Old World, with six conserved open reading frames. The complete nucleotide sequence of the DNA-A molecule of the present isolate was 2728 nucleotides in length, having the highest sequence identity (83.5%) with an Indian begomovirus causing cotton leaf curl disease. It thus belongs to a novel geminivirus species, and the name Mesta yellow vein mosaic virus is proposed.
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