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Analysis of Brain Mitochondria Using Serial Block-Face Scanning Electron Microscopy
Published on: July 9, 2016
Intense exercise induces mitochondrial dysfunction in mice brain
Aderbal S Aguiar1, Talita Tuon, Cléber A Pinho
1Laboratory of Exercise Physiology and Biochemistry, Post-graduation Program in Health Sciences, Universidade do Extremo Sul Catarinense, Criciúma, SC 88806-000, Brazil.
Neurochemical Research
|July 10, 2007
Summary
Intense exercise negatively impacts brain health by reducing brain-derived neurotrophic factor (BDNF) and increasing oxidative stress. This study reveals potential mitochondrial dysfunction from high-intensity training in mice.
Area of Science:
- Neuroscience
- Exercise Physiology
- Mitochondrial Biology
Background:
- Exercise-induced free radicals can impact neurotrophins and brain oxidative metabolism.
- Understanding these effects is crucial for optimizing training protocols and brain health.
Purpose of the Study:
- To investigate the impact of intense physical training on brain-derived neurotrophic factor (BDNF) levels.
- To assess changes in cyclooxygenase (COX) activity and lipoperoxidation in the mouse brain cortex following exercise.
Main Methods:
- Adult male CF1 mice were divided into control, intermittent exercise, and continuous exercise groups.
- High-intensity exercise was confirmed by blood lactate levels.
- Measurements included citrate synthase activity, COX activity, BDNF levels, and thiobarbituric acid reactive species (TBARS).
Main Results:
- Intense exercise significantly increased citrate synthase activity, indicating enhanced mitochondrial capacity.
- Intermittent training reduced brain cortex COX activity, while BDNF levels decreased in both exercise groups.
- Both exercise protocols elevated TBARS levels, suggesting increased oxidative stress in the brain cortex.
Conclusions:
- Intense exercise, both continuous and intermittent, may lead to brain mitochondrial dysfunction.
- Decreased BDNF levels in the frontal cortex appear to be a key factor in exercise-induced brain changes.
- Further research is needed to elucidate the long-term consequences of exercise-induced oxidative stress on neurotrophic factors.
