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[Detecting ABO blood type of bloodstain with fluorescent antibody method]
Rui-Heng Wang1, Li-Qiang Yan, Wei-Jian Yu
1Law School of Liaoning Normal University, Dalian 116029, China.
Unlabelled:
OBJECTIVE To explore the advantage and feasibility of fluorescent antibody method for detection of blood type in biological material.
Methods:
According to theory of specific binding of antigen and antibody, at first the anti-A monoclonal antibody (MA) and anti-B MA were labeled with the fluorescent, then fluorescent-labeled antibodies (FLA) were bound with corresponding biological material (such as bloodstain) in the optimum condition, finally the ABO blood type of bloodstain was determined under microscope fluorescent.
Results:
The fluorescent antibody method is highly sensitive, accurate and simple.
Conclusion:
The fluorescent antibody method is an accurate and reliable method for detection of ABO blood type in biological material.
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