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Published on: December 28, 2015
Chemoenzymatic approaches to glycoprotein synthesis.
Clay S Bennett1, Chi-Huey Wong
1Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.
Chemical Society Reviews
|July 11, 2007
Summary
Synthesizing homogeneous glycoproteins is challenging. Recent advances in ligation technologies and glycan introduction methods now enable the creation of precisely engineered glycoproteins.
Area of Science:
- Glycochemistry
- Chemical Biology
- Synthetic Chemistry
Background:
- Homogeneous glycoprotein synthesis is a significant challenge in chemistry.
- Recent years have seen rapid development in synthetic methodologies.
- These methods address the precise introduction of glycans onto peptide backbones.
Purpose of the Study:
- To review recent advancements in glycoprotein synthesis.
- To discuss techniques for creating well-defined homogeneous glycoproteins.
- To highlight novel ligation and glycan introduction strategies.
Main Methods:
- Novel ligation technologies for protein backbone synthesis.
- Chemical methods for complex glycan introduction.
- Enzymatic approaches for glycan conjugation.
Main Results:
- A growing toolkit of methods for glycoprotein synthesis is now available.
- These techniques allow for precise control over glycan structures.
- The synthesis of homogeneous glycoproteins is becoming more accessible.
Conclusions:
- The field of homogeneous glycoprotein synthesis has advanced significantly.
- Synthetic and enzymatic strategies offer powerful tools for researchers.
- These advancements facilitate the study and application of well-defined glycoproteins.
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