Related Experiment Video
Updated: Jul 13, 2026

Applying Hyperspectral Reflectance Imaging to Investigate the Palettes and the Techniques of Painters
Published on: June 18, 2021
Raman spectra of proteinaceous materials used in paintings: a multivariate analytical approach for classification and
Austin Nevin1, Iacopo Osticioli, Demetrios Anglos
1Department of Chemistry, University of Florence, Polo Scientifico e Tecnologico, via della Lastruccia 3, I-50019, Firenze, Italy. austin.nevin@courtauld.ac.uk
Abstract:
This work presents Raman spectra obtained from thin films of protein materials which are commonly used as binding media in painted works of art. Spectra were recorded over the spectral range of 3250-250 cm(-1), using an excitation wavelength of 785 nm, and several bands have been identified in the fingerprint region that correspond to the various proteins examined. Differences in the C-H vibrations located between 3200 and 2700 cm(-1) can be accounted for with reference to the amino acid composition of the protein-based binding media as well as the presence of fatty acid esters, in the case of egg yolk. In addition, the discrimination of different proteins on the basis of variations in spectra between 3200 and 2700 cm(-1) can be achieved following multivariate analysis of a large data set of spectra, providing a novel and nondestructive alternative based on Raman spectroscopy to other methods commonly used for the analysis of proteins.
Related Concept Videos
Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Rapid Identification of Pathogens
Mass Spectrum: Interpretation
NMR Spectroscopy of Aromatic Compounds
Spectroscopy of Carboxylic Acid Derivatives
In the...
