Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Methods of Classification and Identification01:28

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
Human Virome01:26

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-world emergence of nirsevimab resistance in breakthrough infections with respiratory syncytial virus-B: a multicentre observational study in France.

The Lancet. Microbe·2026
Same author

Ultrasound-assisted valorization of Posidonia oceanica polysaccharides: Discovery of novel monosaccharides and enhanced multifunctional bioactivity.

International journal of biological macromolecules·2026
Same author

Tick-borne encephalitis emergence as the third leading cause of encephalitis/meningoencephalitis in western Auvergne-Rhône-Alpes, France.

Infectious diseases now·2026
Same author

Interplay Between Oral Microbiota and Mouth Health in People Living With HIV Under Antiretroviral Therapy With or Without Periodontitis.

International journal of dentistry·2026
Same author

How multidisciplinary teams matter in public health expertise: A case study on the 2023 French infectious disease prioritization exercise.

Public health·2026
Same author

A new antiviral isorhamnetin glycoside from the Tunisian plant Herniaria hirsuta L.

Fitoterapia·2026

Related Experiment Video

Updated: Jul 13, 2026

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
11:29

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Published on: December 3, 2011

Basic rationale, current methods and future directions for molecular typing of human enterovirus.

Dorsaf Nasri1, Lamjed Bouslama, Sylvie Pillet

  • 1Laboratory of Bacteriology-Virology, GIMAP EA3064, Faculté de Médicine Jacques Lisfranc, Saint-Etienne cedex 02, France. nasridorsaf@yahoo.fr

Expert Review of Molecular Diagnostics
|July 11, 2007
PubMed
Summary

Enteroviruses, a diverse group of viruses, are classified by their structural proteins. Molecular typing methods help understand enterovirus diversity and evolution, with microarrays poised to advance future analysis.

More Related Videos

Particle Agglutination Method for Poliovirus Identification
07:06

Particle Agglutination Method for Poliovirus Identification

Published on: April 20, 2011

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
12:57

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

Published on: November 16, 2017

Related Experiment Videos

Last Updated: Jul 13, 2026

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses
11:29

Cost-effective Method for Microbial Source Tracking Using Specific Human and Animal Viruses

Published on: December 3, 2011

Particle Agglutination Method for Poliovirus Identification
07:06

Particle Agglutination Method for Poliovirus Identification

Published on: April 20, 2011

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
12:57

Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity

Published on: November 16, 2017

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • Enterovirus is a genus in the Picornaviridae family, encompassing over 80 serotypes across four species (Human enterovirus A-D).
  • Structural protein antigens are key to subdividing enteroviruses into distinct serotypes.
  • Understanding enterovirus diversity and evolution is crucial for public health.

Purpose of the Study:

  • To review current molecular typing methods for enteroviruses.
  • To explore the role of comparative phylogeny in enterovirus classification and diversity studies.
  • To highlight the potential of future technologies like microarrays in enterovirus research.

Main Methods:

  • Comparative phylogeny based on molecular typing.
  • Sequencing of amplicons targeting capsid protein regions (VP1, VP2, or VP4).
  • Analysis of both cell culture-derived strains and direct amplification from clinical/environmental specimens.

Main Results:

  • Molecular typing, particularly sequencing of capsid protein genes, is effective for classifying enteroviruses.
  • Phylogenetic analysis aids in investigating enterovirus diversity and evolutionary mechanisms.
  • Current methods allow typing from various sample sources, including direct amplification.

Conclusions:

  • Molecular typing and phylogenetic analysis are essential tools for understanding enterovirus diversity and classification.
  • Future technologies like microarrays are expected to significantly enhance enterovirus typing and virulence factor analysis.
  • Further research into virulence determinants is needed to explain the varied pathologies of human enterovirus infections.