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[von Willebrand factor multimer analysis using electroblotting and immunoperoxidase staining method]
Y Yasumuro1, Y Tomita, T Meguro
1Clinical Laboratory, St. Marianna University School of Hospital, Kawasaki.
Further modification of the previously reported peroxidase staining method for von Willebrand factor (vWF) multimer is described in this article. The alteration are as follows: affinity purified anti-human vWF rabbit IgG was replaced by commercial anti-human vWF rabbit IgG, and washing and reaction steps were shortened markedly. Despite these modification, the resolution of vWF multimers on the nitrocellulose membrane remains comparable to the autoradiography. However, unlike autoradiography, results produced by this method can be obtained in a day. The features of this method are especially useful in clinical laboratories where both time and equipments are limited.
Further modification of the previously reported peroxidase staining method for von Willebrand factor (vWF) multimer is described in this article. The alteration are as follows: affinity purified anti-human vWF rabbit IgG was replaced by commercial anti-human vWF rabbit IgG, and washing and reaction steps were shortened markedly. Despite these modification, the resolution of vWF multimers on the nitrocellulose membrane remains comparable to the autoradiography. However, unlike autoradiography, results produced by this method can be obtained in a day. The features of this method are especially useful in clinical laboratories where both time and equipments are limited.