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Single-Cell Sorting of Immunophenotyped Mesenchymal Stem Cells from Human Exfoliated Deciduous Teeth
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Cell sorting technique based on thermoresponsive differential cell adhesiveness.

Takehisa Matsuda1, Yuko Saito, Kaori Shoda

  • 1Division of Biomedical Engineering, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. tmatsuda@neptune.kanazawa-it.ac.jp

Biomacromolecules
|July 12, 2007
PubMed
Summary

This study introduces a novel cell sorting method using thermoresponsive polymers for tissue engineering. The technique achieves high purity of target cells with simple, damage-free manipulation, advancing stem cell applications.

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Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • Cell sorting is crucial for developing engineered tissues using stem cells.
  • Current methods can be complex or cause cell damage.

Purpose of the Study:

  • To develop a new, simple, and damage-free cell sorting method.
  • To utilize thermoresponsive polymers for selective cell adhesion and detachment.

Main Methods:

  • A mixture of poly(N-isopropylacrylamide)-grafted gelatin (PNIPAM-gelatin) and PNIPAM was used for cell culture dishes.
  • Graded cell adhesion was achieved by adjusting the mixing ratio of the thermoresponsive substances.
  • Cell sorting involved detachment at room temperature and replating at 37°C.

Main Results:

  • The method effectively differentiated adhesive potentials of bovine endothelial and smooth muscle cells.
  • Remarkably pure target cells were harvested using sequential detachment and replating.
  • Confocal laser scanning fluorescence microscopy confirmed high cell purity.

Conclusions:

  • This thermoresponsive polymer-based cell sorting method offers simple manipulation with no cell damage.
  • The technique shows significant potential for advancing stem cell and tissue engineering applications.