Stationary phase characterization and method development
1Waters Corporation, Milford, MA 01757, USA. Uwe_Neue@Waters.com
Abstract:
The properties of stationary phases and their characterization methods are reviewed. New and significant developments have occurred in the last few years, and new methods for stationary phase characterization have become available. The characterization methods are discussed, and the differences between the different methods are pointed out. In addition, method development approaches are reviewed, with special emphasis on recent developments that employ multiple parameters in parallel. Also, the renewed interest of temperature as a tool in method development is surveyed.
More Related Videos
09:49Sedimentation Equilibrium of a Small Oligomer-forming Membrane Protein: Effect of Histidine Protonation on Pentameric Stability
Published on: April 2, 2015
10:44Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
Published on: March 28, 2017
Related Concept Videos
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
Chromatographic Methods: Terminology
Chromatographic Methods: Classification
Chromatographic techniques are typically named by...
Analyte Adsorption and Distribution
Ion-Exchange Chromatography
Chromatography: Introduction
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
