Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Composition of the synaptic PSD-95 complex.

Ayse Dosemeci1, Anthony J Makusky, Ewa Jankowska-Stephens

  • 1Laboratory of Neurobiology, NINDS, National Institutes of Health, Bethesda, Maryland 20892, USA.

Molecular & Cellular Proteomics : MCP
|July 12, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AIRI: Predicting Retention Indices and Their Uncertainties Using Artificial Intelligence.

Journal of chemical information and modeling·2024
Same author

Modification of the synaptic cleft under excitatory conditions.

Frontiers in synaptic neuroscience·2023
Same author

AIomics: Exploring More of the Proteome Using Mass Spectral Libraries Extended by Artificial Intelligence.

Journal of proteome research·2023
Same author

Mass Spectral Library Methods for Analysis of Site-Specific N-Glycosylation: Application to Human Milk Proteins.

Journal of proteome research·2022
Same author

MS_Piano: A Software Tool for Annotating Peaks in CID Tandem Mass Spectra of Peptides and N-Glycopeptides.

Journal of proteome research·2021
Same author

Canonical Wnt Signaling Pathway on Polarity Formation of Utricle Hair Cells.

Neural plasticity·2021
Same journal

A temporal phospho-acetylome atlas of human myogenesis identifies coordinated post-translational regulation.

Molecular & cellular proteomics : MCP·2026
Same journal

Temporal proteomic characterization of SARS-CoV-2 infected mouse lungs.

Molecular & cellular proteomics : MCP·2026
Same journal

Platelet proteome links metabolism to reactivity in Essential Thrombocythemia.

Molecular & cellular proteomics : MCP·2026
Same journal

Genetic rescue of disrupted synaptic protein interaction network dynamics following SYNGAP1 reactivation.

Molecular & cellular proteomics : MCP·2026
Same journal

ASAP-ID: Proximity labelling with small tags.

Molecular & cellular proteomics : MCP·2026
Same journal

Proteome profiling reveals NQO2 activity contributing to proteasome inhibitor resistance in multiple myeloma cell lines.

Molecular & cellular proteomics : MCP·2026
See all related articles

Postsynaptic density protein 95 (PSD-95) forms key synaptic complexes. Affinity purification identified 288 core proteins, including receptors and regulators, revealing insights into synaptic plasticity and structure.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Postsynaptic density protein 95 (PSD-95) is a crucial scaffold protein organizing synaptic function.
  • PSD-95 complexes are large and detergent-insoluble, requiring specific purification methods.

Purpose of the Study:

  • To identify and characterize the protein components of PSD-95-based postsynaptic complexes.
  • To understand the functional implications of these complexes in synaptic plasticity.

Main Methods:

  • Affinity purification of PSD-95 complexes using PSD-95 antibody-coated magnetic beads.
  • Liquid chromatography-tandem mass spectrometry (LC/MS/MS) for protein identification and semiquantitative abundance analysis.

Main Results:

Related Experiment Videos

  • Affinity purification yielded a preparation largely depleted of contaminants.
  • Identified 288 proteins within the PSD-95 complex, including NMDA and AMPA receptors, G-protein regulators, and cell adhesion molecules.
  • Highlighted 26 major components, including novel proteins like BRAG1, BRAG2b, and a sterile alpha motif-containing protein (Q8BZM2).

    • Conclusions:

    • The PSD-95 complex is a core structure at the synapse, essential for organizing receptors and signaling molecules.
    • The identified proteins suggest critical roles in actin cytoskeleton reorganization and protein trafficking, impacting spine plasticity.
    • Novel proteins identified may represent new structural or regulatory elements within the postsynaptic density.