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Updated: Jul 13, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
Published on: June 26, 2020
Phenotypic analysis of cellular responses to DNA damage
Helfrid Hochegger1, Shunichu Takeda
1Dep. of Radiation Genetics, Graduate School Medicine, Kyoto University Sakyo-ku, Kyoto, Japan.
Abstract:
Since the establishment of the first mutant DT40 cell line, a large panel of DT40 mutants in DNA repair genes has been created. The systematic phenotypic analysis of these mutants has provided insight in the mechanisms of various DNA repair pathways in vertebrate. Especially, in the field of double strand break repair DT40 cells have contributed to the advance of knowledge. This review will give a brief overview of DNA double strand break repair, and will summarize phenotypes observed in DT40 mutants in these pathways, focusing on the methodological aspects of analysis.
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Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
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