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Stable non-targeted transfection of DT40
1M.R.C. Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Sub-Cellular Biochemistry
|July 13, 2007
Abstract:
The introduction of stably integrated transgenes into cells is a central technique in any system for genetic analysis, in particular for complementation and for overexpression. This protocol makes use of relatively high electroporation energies that result in DNA breaks and which favour non-targeted integration of the construct into the genome.
