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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jul 13, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Preparation of genomic DNA for microarray-based comparative genome hybridization.

Robert Kimmel¹, Amalia Icreverzi, Paul Neiman

¹Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

Sub-Cellular Biochemistry

|July 13, 2007

Summary

This study details methods for preparing genomic DNA for chicken cDNA microarrays to detect DNA copy-number variations. It also presents a specialized technique for identifying DNA amplifications within palindromic structures.

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Last Updated: Jul 13, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

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Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

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A Novel Strategy Combining Array-CGH, Whole-exome Sequencing and In Utero Electroporation in Rodents to Identify Causative Genes for Brain Malformations

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Published on: December 1, 2017

Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

Comparative hybridization on microarrays is crucial for assessing genomic DNA gains and losses.
Various platforms, including those using cDNA probes, facilitate genome-wide DNA copy-number variation analysis.

Purpose of the Study:

To describe genomic DNA preparation for hybridization to chicken cDNA microarrays.
To present array comparative genomic hybridization (array CGH) methods for assessing DNA copy-number differences.
To detail a specialized array CGH application for detecting DNA amplifications within palindromic structures.

Main Methods:

Genomic DNA preparation for hybridization to chicken cDNA probes on glass slide microarrays.
Method 1: Assessing DNA copy-number differences between experimental and normal diploid genomes.
Method 2: Palindrome enrichment and internal controls for distinguishing direct versus inverted long repeat structures using cDNA microarrays.

Main Results:

Established protocols for preparing genomic DNA for array CGH using chicken cDNA microarrays.
Demonstrated the utility of array CGH for detecting DNA copy-number variations.
Developed a method to identify DNA amplifications within palindromic regions.

Conclusions:

The described methods enable robust genome-wide assessment of DNA copy-number changes using cDNA microarrays.
The specialized technique effectively detects DNA amplifications involving complex palindromic structures.
These protocols advance the application of array CGH in genomic research.