Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Colony survival assay.

Laura J Simpson1, Julian E Sale

  • 1M.R.C. Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.

Sub-Cellular Biochemistry
|July 13, 2007
PubMed
Summary
This summary is machine-generated.

This study details methods for DNA damage survival assays in DT40 cells using chemical mutagens, ionizing radiation, and UV irradiation. These assays are crucial for understanding DNA repair networks and epistasis in DT40 cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-fidelity human chromosome transfer and elimination.

Science (New York, N.Y.)·2025
Same author

Protocol for double-crosslinking ChIP-seq to improve data quality and enhance detection of challenging chromatin targets.

STAR protocols·2025
Same author

ecDNA replication is disorganized and vulnerable to replication stress.

Nucleic acids research·2025
Same author

Engineered Receptor Capture Combined with Mass Spectrometry Enables High-Throughput Detection and Quantitation of SARS-CoV-2 Spike Protein.

JACS Au·2025
Same author

Author Correction: FANCD2-FANCI surveys DNA and recognizes double- to single-stranded junctions.

Nature·2025
Same author

DNA replication initiation drives focal mutagenesis and rearrangements in human cancers.

Nature communications·2024
Same journal

Future Directions in Biotechnological and Pharmacological Applications of CAIs.

Sub-cellular biochemistry·2026
Same journal

Industrial and Environmental Applications of Carbonic Anhydrases.

Sub-cellular biochemistry·2026
Same journal

Applications of Carbonic Anhydrase Inhibitors in Arthritis, Neuropathic Pain, Acute Mountain Sickness, and Cerebral Ischemia.

Sub-cellular biochemistry·2026
Same journal

Applications of Carbonic Anhydrase Inhibitors in Neurological Disorders, Mechanisms and Therapeutic Potential.

Sub-cellular biochemistry·2026
Same journal

Carbonic Anhydrase Inhibitors in Oncology.

Sub-cellular biochemistry·2026
Same journal

Therapeutic Applications of Carbonic Anhydrase Inhibitors in Ophthalmology.

Sub-cellular biochemistry·2026
See all related articles

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Epistasis analysis using DNA damaging agents is vital for studying DNA repair networks.
  • This approach has been highly informative in bacterial, yeast, and DT40 cell lines.
  • DT40 cells, being non-adherent, require specific culture conditions for experimental manipulation.

Purpose of the Study:

  • To present established methods for conducting DNA damage survival assays in DT40 cells.
  • To adapt these assays for various DNA damaging agents, including chemical mutagens, ionizing radiation, and ultraviolet irradiation.
  • To facilitate genetic analysis of DNA repair pathways in DT40.

Main Methods:

  • Culturing DT40 cells in methylcellulose-containing viscous medium to restrict cell movement.

Related Experiment Videos

  • Exposing DT40 cells to chemical mutagens.
  • Exposing DT40 cells to ionizing radiation (e.g., gamma rays or X-rays).
  • Exposing DT40 cells to ultraviolet (UV) irradiation.

    • Main Results:

    • Successfully established protocols for DNA damage survival assays in DT40 cells.
    • Demonstrated the applicability of these assays across different DNA damaging agents.
    • Provided a foundation for future epistasis analysis and DNA repair studies in DT40.

    Conclusions:

    • The presented methods enable robust DNA damage survival assays in DT40 cells.
    • These assays are essential for dissecting DNA repair pathways and epistasis in this model system.
    • The findings will advance the understanding of cellular responses to DNA damage.