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Related Concept Videos

What is the Cell Cycle?01:04

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...
What is the Cell Cycle?01:04

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...
What is the Cell Cycle?00:56

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...
What is the Cell Cycle?00:56

What is the Cell Cycle?

The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...
Determining the Plane of Cell Division02:13

Determining the Plane of Cell Division

Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function. 
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division starting...
Determining the Plane of Cell Division02:13

Determining the Plane of Cell Division

Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function. 
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division starting...

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Related Experiment Video

Updated: Jul 13, 2026

Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

2D cell cycle analysis.

Roger Franklin1, Julian E Sale

  • 1M.R.C. Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.

Sub-Cellular Biochemistry
|July 13, 2007
PubMed
Summary

This study presents a method for cell cycle analysis by monitoring DNA synthesis and content. It

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cell cycle analysis is crucial for understanding cellular processes and responses to stimuli.
  • DT40 cells exhibit rapid cell cycling, with a significant portion in S phase.
  • The p53 protein is a key regulator of the G1/S checkpoint, important for cell cycle control.

Purpose of the Study:

  • To establish a method for snapshot cell cycle stage determination in cell populations.
  • To assess cell cycle progression and response to DNA damage.
  • To evaluate the success of cell synchronization techniques.

Main Methods:

  • Simultaneous monitoring of DNA synthesis via bromodeoxyuridine incorporation.
  • Measurement of total cellular DNA content.

More Related Videos

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
08:52

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain
11:00

Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain

Published on: May 19, 2013

Related Experiment Videos

Last Updated: Jul 13, 2026

Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization
08:52

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

Published on: August 16, 2015

Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain
11:00

Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain

Published on: May 19, 2013

  • Application of this method to DT40 cell populations.

    • Main Results:

    • The method provides a snapshot of cell cycle distribution within a population.
    • Asynchronous DT40 cell populations show 50-60% of cells in S phase.
    • The technique is valuable for assessing cell cycle arrest and apoptosis.

    Conclusions:

    • This method offers a reliable way to analyze cell cycle distribution.
    • Caution is advised when interpreting results from p53-deficient DT40 cells due to checkpoint alterations.
    • The technique is applicable for studying cellular responses to DNA damage and evaluating synchronization.