Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Indirect immunofluorescence microscopy.

Frank R Wettey1, Antony P Jackson

  • 1Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK.

Sub-Cellular Biochemistry
|July 13, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural implications of glycosylation on the voltage-gated sodium channel β3-subunit.

Journal of structural biology·2026
Same author

Structural electrobiology: architecture of the bioelectric code.

Open biology·2026
Same author

The voltage-gated sodium channel β3 subunit modulates C6 glioma cell motility independently of channel activity.

Biochimica et biophysica acta. Molecular basis of disease·2025
Same author

Veratridine-Induced Oscillations in Nav 1.7 but Not Nav 1.5 Sodium Channels Are Revealed by Membrane Potential Sensitive Dye.

Membranes·2025
Same author

A novel SCN3B in-frame codon deletion in a Brugada syndrome patient: Implications for disrupted Na<sub>V</sub>1.5 function.

Journal of molecular and cellular cardiology·2025
Same author

Isoform-specific N-linked glycosylation of NaV channel α-subunits alters β-subunit binding sites.

The Journal of general physiology·2024
Same journal

Future Directions in Biotechnological and Pharmacological Applications of CAIs.

Sub-cellular biochemistry·2026
Same journal

Industrial and Environmental Applications of Carbonic Anhydrases.

Sub-cellular biochemistry·2026
Same journal

Applications of Carbonic Anhydrase Inhibitors in Arthritis, Neuropathic Pain, Acute Mountain Sickness, and Cerebral Ischemia.

Sub-cellular biochemistry·2026
Same journal

Applications of Carbonic Anhydrase Inhibitors in Neurological Disorders, Mechanisms and Therapeutic Potential.

Sub-cellular biochemistry·2026
Same journal

Carbonic Anhydrase Inhibitors in Oncology.

Sub-cellular biochemistry·2026
Same journal

Therapeutic Applications of Carbonic Anhydrase Inhibitors in Ophthalmology.

Sub-cellular biochemistry·2026
See all related articles

This protocol details detecting intracellular proteins using indirect immunofluorescence microscopy in DT40 cells. It is crucial for studying the endocytic machinery

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Understanding intracellular protein localization is key to deciphering cellular functions.
  • The endocytic machinery plays a vital role in cellular transport and signaling.

Purpose of the Study:

  • To present a standardized protocol for detecting intracellular proteins in DT40 cells.
  • To facilitate the investigation of protein distribution within the endocytic pathway.

Main Methods:

  • Indirect immunofluorescence microscopy was employed.
  • DT40 cell line was utilized as the model system.

Main Results:

  • The protocol enables reliable detection of intracellular proteins.
  • The method has been successfully applied to study endocytic machinery proteins.

Related Experiment Videos

Conclusions:

  • This protocol provides a robust method for visualizing intracellular protein localization.
  • It is a valuable tool for cell biology research, particularly in studying endocytosis.