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Updated: Jul 13, 2026

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Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
Published on: April 7, 2023
Isolation of nuclear and cytoplasmic proteins from DT40 cells
Yan-Dong Wang1, Randolph B Caldwell
1GSF, Institute for Molecular Radiobiology, Ingolstaedter Landstr. 1, D-85764 Neuherberg-Munich, Germany.
Sub-Cellular Biochemistry
|July 13, 2007
Abstract:
Nuclear and cytoplasmic proteins from DT40 B cells are extracted by serial fractionation in this protocol. The protein extracts are suitable for the detection of DNA-protein interaction, protein-protein interaction, DNase I footprinting analysis, and related techniques. This is a general protocol that was adapted as shown here to our use due to the fragile nature of DT40 cells and their nuclei.

