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Adenovirus-mediated expression of cyclooxygenase-2 antisense reverse abnormal genetic profile of human adhesion
Ghassan M Saed1, Ayman Al-Hendy, Salama A Salama
1Department of Obstetrics and Gynecology, C.S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, Michigan 48201, USA. gsaed@med.wayne.edu
Objective:
To determine the effects of blocking the translation of cyclooxygenase-2 (COX-2) mRNA on the mRNA levels of type I collagen, type III collagen, fibronectin, and transforming growth factor-beta (TGF-beta1) in fibroblasts obtained from normal peritoneal and adhesion tissues.
Design:
Prospective experimental study.
Setting:
University medical center.
Patient(S):
Fibroblasts established from peritoneal and adhesion tissue of the same patients.
Intervention(S):
Adenovirus with COX-2 treatment of the primary cultured fibroblasts.
Main Outcome Measure(S):
Fibroblasts of normal peritoneal and adhesion tissues were isolated from the same patients. Adhesion and normal peritoneal fibroblasts were transfected with an adenovirus COX-2 mRNA in sense or antisense orientation. RNA was extracted from each treatment and subjected to real time reverse transcriptase-polymerase chain reaction to quantify change in mRNA levels of type I collagen, type III collagen, fibronectin, and TGF-beta1.
Result(S):
Type I collagen, type III collagen, fibronectin, and TGF-beta1 mRNAs were present in both normal peritoneal and adhesion fibroblasts with significantly higher levels in adhesion fibroblasts. Normal fibroblasts transfected with the COX-2 sense virus exhibited an elevated mRNA level in those molecules that reached the mRNA levels seen for adhesion fibroblasts. There were no effects seen on the mRNA levels in those molecules when adhesion fibroblasts were transfected with COX-2 sense virus. Normal fibroblasts transfected with COX-2 antisense virus exhibited no difference in mRNA levels in those molecules as compared with untransfected controls. In contrast, adhesion fibroblasts transfected with COX-2 antisense exhibited markedly reduced mRNA levels in those molecules to levels that were seen in normal peritoneal fibroblasts.
Conclusion(S):
Our data suggest that inhibition of COX-2 may reduce the development of postoperative adhesions by preventing the formation of the adhesion phenotype.
Insights
Blocking cyclooxygenase-2 (COX-2) mRNA translation reduces adhesion fibroblast markers. This suggests inhibiting COX-2 may prevent postoperative adhesions by altering the adhesion phenotype.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Postoperative adhesions are a significant clinical complication.
- Fibroblasts play a key role in adhesion formation.
- Cyclooxygenase-2 (COX-2) is implicated in inflammatory processes and tissue remodeling.
Purpose of the Study:
- To investigate the impact of inhibiting cyclooxygenase-2 (COX-2) mRNA translation on key extracellular matrix components in fibroblasts.
- To compare the effects in fibroblasts from normal peritoneal tissue versus adhesion tissue.
Main Methods:
- Fibroblasts were isolated from normal peritoneal and adhesion tissues of the same patients.
- Cells were transfected with adenovirus carrying COX-2 mRNA in either sense or antisense orientation.
- Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) quantified mRNA levels of type I collagen, type III collagen, fibronectin, and TGF-beta1.
Main Results:
- Adhesion fibroblasts exhibited significantly higher baseline mRNA levels for type I collagen, type III collagen, fibronectin, and TGF-beta1 compared to normal fibroblasts.
- Transfection of normal fibroblasts with COX-2 sense adenovirus increased these mRNA levels to those seen in adhesion fibroblasts.
- Transfection of adhesion fibroblasts with COX-2 antisense adenovirus markedly reduced these mRNA levels to those observed in normal fibroblasts.
Conclusions:
- Inhibition of COX-2 translation in adhesion fibroblasts normalizes the expression of key matrix proteins.
- These findings suggest that targeting COX-2 could be a therapeutic strategy to prevent the formation of postoperative adhesions.
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