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Published on: November 18, 2014

The peptidyl prolyl cis/trans isomerase Pin1 downregulates the Inhibitor of Apoptosis Protein Survivin

P Dourlen1, K Ando, M Hamdane

  • 1Inserm, U837, Place de Verdun, 59045 Lille cedex, France.

Biochimica Et Biophysica Acta
|July 13, 2007
PubMed

Insights

The peptidyl prolyl cis-trans isomerase Pin1 regulates the levels of Inhibitor of Apoptosis Protein Survivin in cancer cells. This Pin1-Survivin interaction impacts mitosis control and cancer mechanisms.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • Pin1 and Survivin are key proteins implicated in cancer.
  • Both proteins are involved in critical cellular processes like apoptosis and mitosis.
  • A functional relationship between Pin1 and Survivin has not been previously established.

Purpose of the Study:

  • To investigate the functional relationship between Pin1 and Survivin.
  • To determine if Pin1 regulates Survivin levels and cellular localization.
  • To elucidate the mechanism of Pin1-mediated Survivin regulation.

Main Methods:

  • Overexpression and silencing of Pin1 in neuroblastoma and COS cells.
  • Immunocytochemistry for co-localization studies.
  • Co-immunoprecipitation to detect protein complex formation.
  • RT-PCR and mutagenesis to investigate regulatory mechanisms.

Main Results:

  • Pin1 overexpression decreased Survivin levels in neuroblastoma cells.
  • Pin1 and Survivin partially co-localized in interphase and mitotic cells.
  • A Pin1/Survivin protein complex was identified.
  • Survivin downregulation by Pin1 occurred at the protein level and depended on Pin1 binding.
  • Pin1 silencing reciprocally increased Survivin levels.

Conclusions:

  • A functional and reciprocal relationship exists between Pin1 and Survivin.
  • Pin1 negatively regulates Survivin protein levels, potentially through a protein complex.
  • Understanding this interaction is crucial for cancer mechanisms and mitosis control.

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