Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Integrins01:10

Integrins

Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Activation of Integrins01:15

Activation of Integrins

Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A portable, ultra-low cost, open-source, pedal-controlled microinjector for laboratory use.

PloS one·2026
Same author

TIE2 links MEKK3-KLF2/4 and PI3K signaling in cerebral cavernous malformation.

The Journal of experimental medicine·2026
Same author

Dll4 assembles the umbilical cord and placental vasculature.

JCI insight·2026
Same author

Artery formation in the intestinal wall and mesentery by intestine-derived Esm1+ endothelial cells.

Nature communications·2025
Same author

Hemodynamic forces prevent myxomatous valve disease in mice through KLF2/4 signaling.

The Journal of clinical investigation·2025
Same author

Lineage tracing studies suggest that the placenta is not a de novo source of hematopoietic stem cells.

PLoS biology·2025

Related Experiment Video

Updated: Jul 13, 2026

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers
05:43

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

Published on: November 8, 2024

Platelet integrins and immunoreceptors.

Ana Kasirer-Friede1, Mark L Kahn, Sanford J Shattil

  • 1Department of Medicine, University of California, San Diego, La Jolla, CA 92093-0726, USA. sshattil@ucsd.edu

Immunological Reviews
|July 13, 2007
PubMed
Summary

Platelet integrins and immunoreceptors are crucial for blood clot formation. Their interactions influence platelet responses to vascular injury, impacting hemostasis and thrombosis.

Area of Science:

  • Hematology
  • Molecular Biology
  • Cell Biology

Background:

  • Platelet adhesion and thrombus formation rely on integrin alphaIIbbeta3 (GPIIb-IIIa).
  • Other platelet integrins (alphaVbeta3, alpha2beta1, alpha5beta1, alpha6beta1) may also contribute to responses during vascular injury.
  • Platelet immunoreceptors possess extracellular immunoglobulin domains and/or intracellular ITAM or ITIM motifs.

Purpose of the Study:

  • To review the structure and function of platelet integrins and immunoreceptors.
  • To explore the functional relationships between these receptor classes.
  • To discuss the impact of their interactions on platelet function in hemostasis and thrombosis.

Main Methods:

  • Literature review of platelet integrin and immunoreceptor research.

More Related Videos

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs
04:15

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs

Published on: February 2, 2024

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
04:32

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

Published on: June 5, 2019

Related Experiment Videos

Last Updated: Jul 13, 2026

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers
05:43

An In Vitro Assay to Study Platelet Migration Using RGD-Functionalized Avidin-Biotin Tethers

Published on: November 8, 2024

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs
04:15

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs

Published on: February 2, 2024

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
04:32

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

Published on: June 5, 2019

  • Analysis of structural and functional data for these receptors.
  • Synthesis of information on receptor interactions and their consequences.

    • Main Results:

    • Integrin alphaIIbbeta3 is central to platelet adhesion and thrombus formation.
    • Multiple integrins and immunoreceptors are expressed on platelets.
    • ITAM-bearing receptors (e.g., FcgammaRIIA, GPVI-FcRgamma) activate integrins, while ITIM-bearing receptors (e.g., PECAM-1) may inhibit them.

    Conclusions:

    • Interactions between platelet integrins and immunoreceptors are critical for regulating platelet responses.
    • These interactions play a significant role in both normal hemostasis and pathological thrombosis.
    • Understanding these relationships provides insights into potential therapeutic targets.