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Related Experiment Video

Updated: Jul 13, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
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Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

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Identification of novel genes expressed during mouse tooth development by microarray gene expression analysis.

Trevor J Pemberton1, Fang-Yuan Li, Shoji Oka

  • 1Institute for Genetic Medicine, University of Southern California, Los Angeles, California 90033, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|July 13, 2007
PubMed
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Researchers identified novel genes crucial for tooth development using gene expression analysis in mice. Five new genes specific to craniofacial tooth development were discovered, advancing our understanding of dental biogenesis.

Area of Science:

  • Developmental Biology
  • Genetics
  • Molecular Biology

Background:

  • Tooth development involves complex genetic regulation.
  • Identifying novel genes is crucial for understanding dental biogenesis and potential developmental disorders.

Purpose of the Study:

  • To discover previously unknown genes involved in tooth development.
  • To identify novel molecular players in the biogenesis of the developing mouse molar tooth (DMT).

Main Methods:

  • Microarray gene expression analysis of the DMT from postnatal day 1 to 10.
  • Comparison with 16 control tissues to identify differentially expressed genes.
  • Validation using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and mRNA in situ hybridization.

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Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
08:14

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

Published on: May 1, 2014

Related Experiment Videos

Last Updated: Jul 13, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
08:14

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

Published on: May 1, 2014

Main Results:

  • Out of the top 100 upregulated genes in the DMT, 29 were previously linked to tooth development.
  • 71 novel genes showed differential expression, with 29 confirmed by qRT-PCR.
  • Five genes (Rspo4, Papln, Amtn, Gja1, Maf) were found to be specific to the developing tooth in the craniofacial region.

Conclusions:

  • This study identified several novel genes critical for tooth development.
  • Five genes show high specificity for craniofacial tooth development, offering new targets for research.
  • The findings expand the known genetic landscape of dental biogenesis.